Journal: Scientific Reports
Article Title: Obesity reduces the anticancer effect of AdipoRon against orthotopic pancreatic cancer in diet-induced obese mice
doi: 10.1038/s41598-021-82617-2
Figure Lengend Snippet: Effects of AdipoRon and obesity-related factors on the survival of Panc02-Luc-ZsGreen cells. ( A ) qRT-PCR analysis of the expression of AdipoRs and LepRs. ( B ) Effects of AdipoRon on the activation of AMPK, p38 MAPK, ERK1/2 and STAT3. The cells were incubated with 25 μg/ml AdipoRon for the indicated times. The uncropped images are shown in Supplementary Fig. S11-1. ( C ) Cell survival. The cells were incubated with various concentrations of AdipoRon for 2 days. The MTT assay and trypan blue dye exclusion test were used for the measurements of cell survival and viability, respectively. The value for each point represents triplicate measurements. ( D ) Cell morphology. The cells were cultured with 30 μg/ml AdipoRon for 2 days. White arrows indicate the cells showing cytoplasmic swelling with large pieces blebbing from the plasma membrane. ( E ) Effect of z-VAD-fmk on AdipoRon-induced cell growth inhibition. The cells were treated with z-VAD-fmk (50 μM) in the presence or absence of AdipoRon (25 μg/ml) for 2 days. ( F ) Effect of U0126 on AdipoRon-induced cell growth inhibition. The cells were treated with U0126 (10 μM) in the presence or absence of AdipoRon (25 μg/ml) for 2 days. ( G ) Effect of adiponectin on cell survival. The cells were incubated with 10 μg/ml or 20 μg/ml adiponectin (APN) and 25 μg/ml AdipoRon for 2 days. ( H ) Effect of glucose on AdipoRon-induced cell growth inhibition. The cells were cultured in low-glucose (1 mg/ml glucose) or high-glucose (4.5 mg/ml) medium containing 10% FBS in the presence or absence of 25 μg/ml AdipoRon. ( I ) Effect of insulin on AdipoRon-induced cell growth inhibition. The cells were incubated with various concentrations of insulin in high-glucose DMEM containing 10% FBS in the presence or absence of 25 μg/ml AdipoRon. ( J ) Effect of IGF-1 on AdipoRon-induced cell growth inhibition. The cells were incubated with various concentrations of IGF-1 in high-glucose DMEM containing 10% FBS in the presence or absence of 25 μg/ml AdipoRon. Cell survival and cell viability were measured with MTT assay and trypan blue staining, respectively. *P < 0.05, **P < 0.01, ns, not significant.
Article Snippet: Murine recombinant leptin and murine recombinant adiponectin were obtained from PeproTech (Rocky Hill, NJ, USA).
Techniques: Quantitative RT-PCR, Expressing, Activation Assay, Incubation, MTT Assay, Cell Culture, Clinical Proteomics, Membrane, Inhibition, Staining
